Background:

The Pax family encodes transcription factors that function during embryogenesis and regulate the temporal and position-dependent differentiation of cells. Pax-8 is expressed in the developing and adult thyroid, the developing secretory system and at lower levels, in the adult kidney. Pax-8 complexes with TTF-1 and TTF-2 to induce thyroid follicular cell differentiation and thyroid hormone biosynthesis by regulating the expression of sodium iodide symporter (NIS), thyroid peroxidase (TPO), thyroglobulin (TG) and the thyrotropin receptor (TSHR). Treatment of FRTL-5 cells with TGFβ1 decreases Pax-8 mRNA levels and Pax-8 DNA binding activity, which suppresses the expression of TG and the formation of thyrocytes. Patients who have autosomal dominant mutations of the Pax-8 gene develop thyroid dysgenesis. The Pax-8 gene produces six isoforms, A to F, that are generated by alternative splicing and differ in their carboxy-terminal regions. The Pax-8 isoforms display different DNA binding capacities and are thought to be functionally distinct. The gene which encodes Pax-8 maps to human chromosome 2q12-q14.

Specificity:

Pax8 polyclonal antibody detects endogenous levels of Pax8 protein.

Alternative names:

PAX8, 2-cyclic phosphate 2-phosphohydrolase, 35-alpha calcimedin, Annexin A3, Annexin III, Annexin-3, ANX3, ANXA 3, Anxa3, ANXA3, Calcimedin 35 alpha, Inositol 1 2 cyclic phosphate 2 phosphohydrolase, Inositol 1, Lipocortin III, OTTHUMP00000160701, OTTHUMP00000219237, OTTHUMP00000219238, PAP III, PAP-III, Placental anticoagulant protein III,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide within Human Pax8 aa 400-450.

Purification & Purity:

Peptide affinity purified.

Molecular weight:

48 kDa

Applications:

WB ICC IHC FC

Usage:

WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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