Background:

Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.

Specificity:

PABPN1 polyclonal antibody detects endogenous levels of PABPN1 protein.

Alternative names:

PABP-2, Poly(A)-binding protein 2, PABII, PABPN1, Nuclear poly(A)-binding protein 1, OPMD, PAB2, PABII, PABP 2, pABP-2, PABP2, PABP2, PABPII, Pabpn1, poly(A) binding protein nuclear 1, Poly(A)-binding protein 2, Poly(A)-binding protein II, PolyA binding protein II, Polyadenylate-binding nuclear protein 1, Polyadenylate-binding protein 2,

Species reactivity:

Human, Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

49 kDa

Applications:

WB ICC/IF IHC FC IP

Usage:

WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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