Background:

DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. The DNA repair proteins Rad51 and Rad52 are key components of the double-strand-break repair (DSBR) pathway. Rad51 is essential for mitotic and meiotic recombination, and its mutation in yeast and mammalian cells results in chromosome loss. Overexpression of Rad52 confers resistance to ionizing radiation and induces homologous intrachromosomal recombination. Rad52 is thought to be involved in an early stage of Rad51-mediated recombination. Additional proteins involved in the pathway include Dmc1 and nibrin. Dmc1 is specifically involved in meiotic recombination. Nibrin, which complexes with Mre11 and Rad50, is absent in Nijemegen breakage syndrome (NBS) patients.

Specificity:

p95 NBS1 polyclonal antibody detects endogenous levels of p95 NBS1 protein.

Alternative names:

NBN, AT V1, AT V2, ATV, Cell cycle regulatory protein p95, FLJ10155, MGC87362, Nbn, NBN, NBS 1, NBS, NBS1, Nibrin, Nijmegen breakage syndrome 1 (nibrin), Nijmegen breakage syndrome, Nijmegen breakage syndrome protein 1, p95, p95 protein of the MRE11/RAD50 complex,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

95 kDa

Applications:

WB ICC/IF IP FC IHC CHIP

Usage:

WB:1:1,000 ICC:1:50-1:200 FC:1:50-1:100 IHC:1:50

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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