Background:

Found in both primary and transformed human cells, paraspeckles are discrete bodies in the interchromatin nucleoplasmic space which contain p54/nrb (nuclear RNA-binding protein) and at least two other RNA-binding proteins, paraspeckle protein 1 (PSP1) and paraspeckle protein 2 (PSP2). Paraspeckles often co-localize with splicing speckles, which are the site of splicing factor accumulation. Paraspeckle proteins, including p54/nrb, move dynamically between the nucleolus and paraspeckles and translocate to distinct caps in the nucleolar periphery when transcription is inhibited. Originally purified from HeLa cells, the nuclear p54/nrb has two RNA recognition motifs and shares extensive homology with both the human splicing factor PSF and Drosophila NONA/BJ6, which is required for normal vision and courtship. The shared domain between these proteins is termed a DBHS (Drosophila behavior, human splicing) domain and may play a role in regulating various pathways at the level of pre-mRNA splicing.

Specificity:

p54nrb polyclonal antibody detects endogenous levels of p54nrb protein.

Alternative names:

NonO protein, p54nrb, NONO, NRB54, 52 kDa subunit, 54 kDa nuclear RNA and DNA binding protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, DNA binding p52/p100 complex 52 kDa subunit, DNA-binding p52/p100 complex, NMT 55, NMT55, Non Pou domain containing octamer (ATGCAAAT) binding protein, Non POU domain containing octamer binding, Non POU domain containing octamer binding protein, Non-POU domain-containing octamer-binding protein, Nono, NonO protein, NONO, NRB 54, NRB, NRB54, Nuclear RNA binding protein 54kD, P54, p54(nrb), p54nrb, PPP1R114, Protein phosphatase 1 regulatory subunit 114,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein of C-terminal human p54nrb.

Purification & Purity:

ProA affinity purified

Molecular weight:

54 kDa

Applications:

WB ICC IF IHC

Usage:

WB:1:500-1:2,000 ICC:1:50 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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