Background:

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.

Specificity:

Nuf2 polyclonal antibody detects endogenous levels of Nuf2 protein.

Alternative names:

hNuf2, hNuf2R, hNuf2R, Cancer/testis antigen 106, CDCA 1, CDCA1, Cell division cycle associated 1, Cell division cycle associated protein 1, Cell division cycle-associated protein 1, CT106, hNuf 2, hNuf2, hNuf2R, hsNuf2, Kinetochore protein Nuf2, Nuf 2, NUF2, NUF2 NDC80 kinetochore complex component, NUF2 NDC80 kinetochore complex component homolog, NUF2, S. cerevisiae, homolog of, NUF2, NUF2R,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within human Nuf2 aa 1-200.

Purification & Purity:

ProA affinity purified

Molecular weight:

54 kDa

Applications:

WB FC

Usage:

WB:1:500-1:2,000 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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