Background:

Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARTNL/BMAL1, CLOCK and CRY1. Also regulates genes involved in metabolic functions, including lipid and bile acid metabolism, adipogenesis, gluconeogenesis and the macrophage inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-[A/G]GGTCA-3' preceded by an A/T-rich 5' sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two nucleotides (RevDR-2).

Specificity:

NR1D1 polyclonal antibody detects endogenous levels of NR1D1 protein.

Alternative names:

EAR-1, NR1D1, EAR1, HREV, THRAL, EAR-1, EAR1, ERBA-related 1, hRev, Nr1d1, NR1D1, Nuclear receptor Rev ErbA alpha, Nuclear receptor subfamily 1 group D member 1, Rev erbAalpha, Rev erbalpha, Rev-erbA-alpha, Rev-ErbAalpha, Reverba, THRA1, THRAL, Thyroid hormone receptor, alpha like, Thyroid hormone receptor, alpha-1- like, V-erbA-related protein 1,

Species reactivity:

Human, Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

67 kDa

Applications:

WB IHC

Usage:

WB:1:500-1:1,000 IHC:1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com