Background:

Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.

Specificity:

nNOS polyclonal antibody detects endogenous levels of nNOS protein.

Alternative names:

N-NOS, nNOS, NOS1, 2310005C01Rik, BNOS, Constitutive NOS, EC 1.14.13.39, IHPS 1, IHPS1, N-NOS, NC-NOS, neuronal Nitric Oxide Synthase, Neuronal NOS, Nitric oxide synthase , neuronal, included, Nitric oxide synthase 1 (neuronal), Nitric oxide synthase 1, Nitric oxide synthase, brain, Nitric oxide synthase, penile neuronal, included, NNOS, NO, NOS 1, NOS, NOS type I, NOS-I, NOS1, NOS1, Peptidyl-cysteine S-nitrosylase NOS1,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

161 kDa

Applications:

WB ICC/IF IHC IP FC

Usage:

WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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