Background:

Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes MyoD, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either MyoD or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. MyoD transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. MyoD-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.

Specificity:

MyoD1 polyclonal antibody detects endogenous levels of MyoD1 protein.

Alternative names:

bHLHc1, Myf-3, MYOD1, bHLHc1, Class C basic helix-loop-helix protein 1, MYF 3, Myf-3, MYF3, Myoblast determination protein 1, Myod 1, MYOD, MYOD1, MYOD1, Myogenic differentiation 1, Myogenic factor 3, Myogenic factor MYF 3, Myogenin D1, PUM,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

45 kDa

Applications:

WB IP FC

Usage:

WB:1:1,000-1:5,000 IP:1:10-1:50 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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