Background:

MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

Specificity:

MYLK polyclonal antibody detects endogenous levels of MYLK protein.

Alternative names:

MLCK, smMLCK, KRP, MYLK, deglutamylated form, DKFZp686I10125, EC 2.7.11.18, FLJ12216, Kinase related protein, Kinase-related protein, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK, MYLK, MYLK1, Myosin light chain kinase, Myosin light polypeptide kinase, OTTHUMP00000180642, OTTHUMP00000180643, smMLCK, smooth muscle, Smooth muscle myosin light chain kinase, Telokin,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

110 kDa

Applications:

WB ICC/IF IHC FC

Usage:

WB:1:2,000-1:10,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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