Background:

Encoded by the MYL2 gene, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, also designated MLC-2 or MLC2v, is part of a hexameric complex of two heavy chains and four light chains predominantly expressed in adult cardiac ventricle muscle. Myosin regulatory light chain 2 binds calcium and has been shown to be a useful molecular marker for cardiac chamber specification. The co-expression of myosin regulatory light chain 7 (MYL7) and myosin regulatory light chain 2 in the outflow tract and atrioventricular canal, together with the single expression in the atrial (MYL7) and ventricular (MYL2) myocardium, permits the delineation of their boundaries. At the amino acid level there is 96% homology between the human and mouse myosin regulatory light chain sequences. Mutations in MYL2 are correlated with mid-left ventricular chamber type hypertrophic cardiomyopathy (MVC2) as well as familial hypertrophic cardiomyopathy type 10 (CMH10).

Specificity:

MYL2 polyclonal antibody detects endogenous levels of MYL2 protein.

Alternative names:

MLC-2, MLC-2v, MLC-2s/v, MYL2, Cardiac myosin light chain-2, Cardiac ventricular myosin light chain 2, CMH10, MLC 2v, MLC-2, MLC-2v, MLC2, MLRV, MYL 2, MYL2, Myosin light chain 2 regulatory cardiac slow, Myosin light polypeptide 2 regulatory cardiac slow, Myosin regulatory light chain 2, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform, Regulatory light chain of myosin, RLC of myosin, Slow cardiac myosin regulatory light chain 2, ventricular/cardiac muscle isoform,

Species reactivity:

Human, Mouse, Rat, zebrafish

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

19 kDa

Applications:

WB IHC IP

Usage:

WB:1:1,000-1:5,000 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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