Background:

Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of myosin light chain by myosin light chain kinase. Myosin heavy chains are encoded by the MYH gene family and have Actin-activated ATPase activity which generates the motor function of myosin. Myosin heavy chains, which were initially isolated from a human fetal skeletal muscle, are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chain are differentially expressed depending on the functional activity of the muscle.

Specificity:

MYH6 polyclonal antibody detects endogenous levels of MYH6 protein.

Alternative names:

MyHC-alpha, MYH6, embryonic, fast skeletal muscle, HEMHC, Muscle embryonic myosin heavy chain 3, Muscle embryonic myosin heavy chain, MYH 3, Myh3, MYH3, MYHC EMB, MYHSE 1, MYHSE1, Myosin heavy chain 3, Myosin heavy chain 3 skeletal muscle embryonic, Myosin heavy chain, Myosin heavy chain fast skeletal muscle embryonic, Myosin Heavy Polypeptide 3, Myosin heavy polypeptide 3 skeletal muscle embryonic, Myosin skeletal heavy chain embryonic 1, Myosin-3, SMHCE,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

224 kDa

Applications:

WB IHC

Usage:

WB:1:1,000-1:2,000 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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