Background:

It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1 (alternatively designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad-binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors.

Specificity:

mSin3A polyclonal antibody detects endogenous levels of mSin3A protein.

Alternative names:

SIN3A, AW553200, DKFZP434K2235, FLJ90319, Histone deacetylase complex subunit Sin 3a, Histone deacetylase complex subunit Sin3a, KIAA0700, Kiaa4126, mKIAA4126, Paired amphipathic helix protein Sin 3a, Paired amphipathic helix protein Sin3a, Sin 3a, SIN3 homolog A, SIN3 homolog A transcription regulator (yeast), SIN3 homolog A transcription regulator, SIN3 transcription regulator homolog A, Sin3a, SIN3A protein, SIN3A, Transcriptional co repressor Sin 3A, Transcriptional co repressor Sin3A, Transcriptional corepressor Sin3a, Transcriptional regulator SIN3A,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

145 kDa

Applications:

WB ICC/IF IHC FC

Usage:

WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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