Background:

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.

Specificity:

Mib1 polyclonal antibody detects endogenous levels of Mib1 protein.

Alternative names:

DIP-1, MIB1, DIP1, KIAA1323, ZZANK2, DAPK-interacting protein 1, Dip 1, DIP-1, Dip1, E3 ubiquitin protein ligase MIB 1, E3 ubiquitin protein ligase MIB1, E3 ubiquitin-protein ligase mib1, KIAA1323, LVNC7, MIB, mib1, MIB1, Mind bomb homolog 1, Mindbomb E3 ubiquitin protein ligase 1, Ubiquitin ligase mind bomb, Zinc finger ZZ type with ankyrin repeat domain protein 2, ZZANK2, ZZZ6,

Species reactivity:

Human, Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within N-terminal human Mib1.

Purification & Purity:

ProA affinity purified

Molecular weight:

110 kDa

Applications:

WB ICC IHC

Usage:

ICC:1:50-1:200 IHC:1:50-1:200 WB:1:500

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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