Background:

MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O (6) position of guanine, restoring guanine to its normal form without causing DNA breaks. MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents. It is ubiquitously expressed in normal human tissues and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy.

Specificity:

MGMT polyclonal antibody detects endogenous levels of MGMT protein.

Alternative names:

MGMT, 6 O methylguanine DNA methyltransferase, 6-O-methylguanine-DNA methyltransferase, Agat, AGT, AI267024, EC 2.1.1.63, Methylated DNA protein cysteine methyltransferase, Methylated-DNAprotein-cysteine methyltransferase, Methylguanine DNA methyltransferase, MGC107020, MGMT, MGMT, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

peptide

Purification & Purity:

Peptide affinity purified

Molecular weight:

22 kDa

Applications:

WB ICC IHC FC

Usage:

WB:1:500-1:1,000 ICC:1:200 IHC:1:200 FC:1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com