Background:

Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.

Specificity:

MGEA5 polyclonal antibody detects endogenous levels of MGEA5 protein.

Alternative names:

OGA, NCOAT, MGEA5, HEXC, KIAA0679, MEA5, Beta-hexosaminidase, Beta-N-acetylglucosaminidase, Beta-N-acetylhexosaminidase, Bifunctional protein NCOAT, HEXC, Hyaluronidase in meningioma, KIAA0679, MEA5, Meningioma expressed antigen 5, Meningioma-expressed antigen 5, MGEA 5, Mgea5, N-acetyl-beta-D-glucosaminidase, N-acetyl-beta-glucosaminidase, NCOAT, Nuclear cytoplasmic O GlcNAcase and acetyltransferase, Nuclear cytoplasmic O-GlcNAcase and acetyltransferase, OGA, OGA, Protein O-GlcNAcase,

Species reactivity:

Human, Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein of N-terminal human MGEA5.

Purification & Purity:

ProA affinity purified

Molecular weight:

103 kDa

Applications:

WB IHC IP

Usage:

WB:1:500-1:2,000 IHC:1:50-1:200 IP:1:10-1:50

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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