Background:

Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T-47D, was shown to complete mitosis in the presence of Nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during pro-metaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with Insulin receptor (IR), but not IGF-IR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and, if the process is not complete, MAD2B delays the onset of anaphase.

Specificity:

Mad2L2 polyclonal antibody detects endogenous levels of Mad2L2 protein.

Alternative names:

MAD2-like protein 2, hREV7, MAD2L2, MAD2B, REV7, Homolog of REV7 S cerevisiae, hREV7, MAD2 (mitotic arrest deficient yeast, homolog) like 2, MAD2 homolog, MAD2 like 2, MAD2 mitotic arrest deficient like 2, MAD2-like protein 2, MAD2B, Mad2l2, MD2L2, Mitotic Arrest Deficient 2 L2, Mitotic arrest deficient 2-like protein 2, Mitotic arrest deficient homolog like 2, Mitotic arrest deficient like 2 (yeast), Mitotic arrest deficient yeast homolog, Mitotic spindle assembly checkpoint protein MAD2B, Polymerase (DNA directed) zeta 2 accessory subunit, POLZ2, REV 7, REV7, REV7 homolog, Weakly similar to Mitotic MAD2 protein (S cerevisiae),

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

24 kDa

Applications:

WB IHC FC IP

Usage:

WB:1:500 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com