Background:

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin.

Specificity:

macroH2A.1 polyclonal antibody detects endogenous levels of macroH2A.1 protein.

Alternative names:

Histone macroH2A1, mH2A1, H2A/y, H2AFY, MACROH2A1, Core histone macro h2a.1, Core histone macro-H2A.1, H2A histone family member Y, H2A.y, H2A/y, H2AF12M, H2AFJ, H2afy, H2AY, Histone H2A.Y, Histone macroH2A1, Histone macroH2A1.1, Histone macroH2A1.2, Macroh2a1, MACROH2A1.1, MacroH2A1.2, Medulloblastoma antigen MU MB 50.205, Medulloblastoma antigen MU-MB-50.205, mH2a, mH2A1,

Species reactivity:

Human, Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within human macroH2A.1 aa 200-400.

Purification & Purity:

Protein affinity purified.

Molecular weight:

40 kDa

Applications:

WB IHC FC

Usage:

WB:1:500 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com