Background:

Histone methylation regulates chromatin structure and transcription and maintains an epigenetic state of the cell. Histone methylation is dynamically regulated by histone methylases and demethylases. Lysine-specific histone demethylase 1 (LSD1), also designated BHC110, is a flavin-dependent amine oxidase which catalyzes the removal of one or two methyl groups from the methyl-lysine-4 side chain of Histone H3. The LSD1 protein contains a SWIRM domain, a FAD-binding motif and an amine oxidase domain. Association with CoREST, a SANT domain-containing corepressor, positively regulates LSD1. CoREST mediates the demethylation ability of LSD1 and protects it from proteasomal degradation in vivo. PHF21A (also designated BCH80), a PHD domain-containing protein, inhibits activity of LSD1/CoREST mediated de-methylation. The LSD1 protein also co-localizes with the androgen receptor in human prostate tumor cells and in unaffected prostate cells, stimulating androgen-receptor-dependent transcription.

Specificity:

LSD1 polyclonal antibody detects endogenous levels of LSD1 protein.

Alternative names:

KDM1A, AOF2, KDM1, KIAA0601, LSD1, Amine oxidase (flavin containing) domain 2, Amine oxidase, flavin containing, 2, AOF2, BHC110, BRAF35 HDAC complex protein BHC110, BRAF35-HDAC complex protein BHC110, BRAF35/HDAC complex, 110-kD subunit, CPRF, EC1, FAD binding protein BRAF35 HDAC complex, 110 kDa subunit, Flavin-containing amine oxidase domain-containing protein 2, KDM 1, KDM1, Kdm1a, KDM1A, KIAA0601, LSD 1, LSD1, Lysine (K) specific demethylase 1, Lysine (K) specific demethylase 1A, Lysine demethylase 1A, Lysine specific histone demethylase 1, Lysine specific histone demethylase 1A, Lysine-specific demethylase 1, Lysine-specific demethylase 1A, Lysine-specific histone demethylase 1A,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Peptide

Purification & Purity:

Peptide affinity purified

Molecular weight:

110 kDa

Applications:

WB ICC IHC

Usage:

WB:1:500-1,000 ICC:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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