Background:

In eukaryotes, it is essential to have the ability to detect and degrade transcripts that lack full coding potential. Nonsense-mediated RNA decay (NMD) protects the organism by avoiding the translation of truncated peptides with dominant negative or deleterious gain-of-function potential. Rent1, a mammalian ortholog of Upflp, is essential for embryonic viability. Rent1 (also designated regulator of nonsense transcripts and HUpf1) contains an N-terminal zinc finger-like domain, NTPase domains and a region comprised of domains that define Rent1 as a superfamily group I helicase. Rent1 protein has nucleic-acid-dependent ATPase activity and 5' to 3' helicase activity. In addition, Rent1 is an RNA-binding protein whose activity is modulated by ATP and directly interacts with Rent2, which is a mammalian homolog of Upf2p. Two mammalian orthologs to Upf3p, Rent3a and Rent3b, are encoded by two separate genes. Rent3b (also known as Rent3X) is encoded by a X-linked gene and localizes primarily to the nucleus, while Rent 1 and Rent 2 localize primarily in the cytoplasm. Specific Rent3 protein interactions with Y14 and spliced mRNA suggest Rent3a and Rent3b serve as a link between splicing and NMD in the cytoplasm.

Specificity:

hUPF1 polyclonal antibody detects endogenous levels of hUPF1 protein.

Alternative names:

NORF1, hUpf1, UPF1, KIAA0221, RENT1, ATP dependent helicase RENT1, ATP-dependent helicase RENT1, Delta helicase, FLJ43809, FLJ46894, HUPF 1, hUpf1, KIAA0221, Nonsense mRNA reducing factor 1, NORF 1, NORF1, pNORF 1, pNORF1, Regulator of nonsense transcripts 1, RENT 1, RENT1, RENT1, Smg 2, Smg 2 homolog nonsense mediated mRNA decay factor, UP Frameshift 1, Up frameshift mutation 1 homolog (S. cerevisiae), Up frameshift mutation 1 homolog, Up frameshift suppressor 1 homolog, Up-frameshift suppressor 1 homolog, UPF 1, UPF 1 regulator of nonsense transcripts homolog, upf1, UPF1 regulator of nonsense transcripts homolog, UPF1 RNA helicase and ATPase, Yeast Upf1p homolog,

Species reactivity:

Human, Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within human hUPF1 aa 1-200.

Purification & Purity:

ProA affinity purified

Molecular weight:

124 kDa

Applications:

WB,IF

Usage:

WB 1:500 - 1:2000 IF 1:50 - 1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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