Background:

High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFκB family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.

Specificity:

HMGB2 polyclonal antibody detects endogenous levels of HMGB2 protein.

Alternative names:

HMG-2, HMGB2, HMG2, C80539, High mobility group (nonhistone chromosomal) protein 2, High mobility group box 2, High mobility group protein 2, High mobility group protein B2, HMG 2, HMG B2, HMG-2, HMG2, HMGB2, HMGB2,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein.

Purification & Purity:

Protein affinity purified.

Molecular weight:

24 kDa

Applications:

WB ICC IHC

Usage:

WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com