Background:

Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.

Specificity:

HIF-1α polyclonal antibody detects endogenous levels of HIF-1α protein.

Alternative names:

HIF-1-alpha, HIF1-alpha, bHLHe78, HIF1A, BHLHE78, MOP1, PASD8, ARNT interacting protein, ARNT-interacting protein, Basic helix loop helix PAS protein MOP1, Basic-helix-loop-helix-PAS protein MOP1, bHLHe78, Class E basic helix-loop-helix protein 78, HIF 1A, HIF 1alpha, HIF-1-alpha, HIF1 A, HIF1 Alpha, HIF1, HIF1-alpha, HIF1A, HIF1A, Hypoxia inducible factor 1 alpha, Hypoxia inducible factor 1 alpha isoform I.3, Hypoxia inducible factor 1 alpha subunit, Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor, Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor), Hypoxia inducible factor1alpha, Hypoxia-inducible factor 1-alpha, Member of PAS protein 1, Member of PAS superfamily 1, Member of the PAS Superfamily 1, MOP 1, MOP1, PAS domain-containing protein 8, PASD 8, PASD8,

Species reactivity:

Human, Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Peptide

Purification & Purity:

Peptide affinity purified

Molecular weight:

93 kDa

Applications:

WB ICC FC

Usage:

WB:1:500 ICC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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