Background:

The Drosophila Hairy and enhancer of split genes encode basic helix-loop-helix (bHLH) transcriptional repressors that function in the Notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homolog of Drosophila Hairy and enhancer of split are the HES gene family members HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homolog of groucho, and this interaction is mediated by the carboxy-terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues.

Specificity:

Hes1 polyclonal antibody detects endogenous levels of Hes1 protein.

Alternative names:

bHLHb39, hHL, HES1, bHLHb39, C-HAIRY1, c-hairy1A, Class B basic helix-loop-helix protein 39, FLJ20408, Hairy and enhancer of split 1 (Drosophila), Hairy and enhancer of split 1, Hairy homolog (Drosophila), Hairy homolog, Hairy like, Hairy, Drosophila, homolog of, Hairy-like protein, Hairy/enhancer of split, Drosophila, homolog of, 1, HAIRY1, HES-1, hes1, Hes1 hairy and enhancer of split 1 (Drosophila), HES1, HHL, HL, HRY, MGC129109, OTTHUMP00000209031, RHL, Transcription factor HES-1,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

30 kDa

Applications:

WB ICC/IF IHC FC

Usage:

WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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