Background:

Gamma-glutamylcysteine synthetase (γ-GCS) is the rate limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. γ-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human gamma-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human γ-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of γ-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.

Specificity:

GCLM polyclonal antibody detects endogenous levels of GCLM protein.

Alternative names:

GCLM, GLCLR, Gamma ECS regulatory subunit, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GCLM, GCS light chain, GLCLR, Glutamate cysteine ligase regulatory subunit, Glutamatecysteine ligase modifier subunit, Glutamatecysteine ligase regulatory subunit, GSC light chain, GSH0,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

Applications:

WB ICC/IF IHC FC IP

Usage:

WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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