Background:

The four-and-a-half-LIM domain (FHL) proteins include FHL-1 (SLIM1), FHL-2 (SLIM3), FHL-3 (SLIM2) and FHL-4. The signature "half-domain", a single zinc finger domain located in the N-terminal region, differentiates FHLs from other LIM-only proteins, which have numbers of zinc fingers. Specific combinations of FHL proteins elicit selective activation of both CREB and CREM. Skeletal and cardiac muscle express FHL-1 in high levels as compared to the low level of expression in smooth muscle of the colon, small intestine and prostate. FHL-1 localizes to the cytosol of myoblasts, myotubes, and differentiated myocytes. FHL-2 is also located in cardiac and skeletal muscle, as well as in placenta and ovary tissues. FHL-3 is found in skeletal muscle, but absent in cardiac muscle. FHL-4 is expressed exclusively by the seminiferous epithelium of the testis, which suggests that FHL-4 is involved in spermatogenesis. The genetic loci for FHLs vary considerably despite similiar amino acid sequences among the FHL group.

Specificity:

FHL2 polyclonal antibody detects endogenous levels of FHL2 protein.

Alternative names:

FHL-2, SLIM-3, FHL2, DRAL, SLIM3, AAG 11, AAG11, Aging associated gene 11, Down regulated in rhabdomyosarcoma LIM protein, Downregulated in rhabdomyosarcoma LIM protein, DRAL, FHL 2, FHL-2, Fhl2, FHL2 protein, FHL2, Four and a half LIM domain protein 2, Four and a half LIM domains 2, Four and a half LIM domains protein 2, KIAA0990, LIM domain protein DRAL, Skeletal muscle LIM protein 3, Skeletal muscle LIM-protein 3, SLIM 3, SLIM-3, SLIM3,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within C-terminal human FHL2.

Purification & Purity:

ProA affinity purified

Molecular weight:

32 kDa

Applications:

WB IHC IP

Usage:

WB:1:500-1:2,000 IHC:1:50-1:200 IP:1:10-1:50

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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