Background:

Emi1 (for early mitotic inhibitor) regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry. Emi1 is required to arrest unfertilized eggs at metaphase of meiosis II and may be the long-sought mediator of CSF activity. Human Emi1 is similar to Xenopus laevis Emi1, which inhibits the APC (Cdc20) ubiquitination complex to allow accumulation of cyclin B.

Specificity:

Emi1 polyclonal antibody detects endogenous levels of Emi1 protein.

Alternative names:

FBXO5, EMI1, FBX5, Early mitotic inhibitor 1, Emi 1, EMI1, F box only protein 5, F box protein 5, F box protein Fbx 5, F box protein Fbx5, F-box only protein 5, FBX5, FBX5, Fbxo31, fbxo5,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within human Emi1 aa 50-250.

Purification & Purity:

ProA affinity purified

Molecular weight:

50 kDa

Applications:

WB ICC IHC

Usage:

ICC:1:50-1:200 IHC:1:50-1:200 WB:1:500

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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