Background:

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA.

Specificity:

ELAVL1 polyclonal antibody detects endogenous levels of ELAVL1 protein.

Alternative names:

HuR, ELAVL1, HUR, ELAV (embryonic lethal abnormal vision Drosophila) like 1, ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R), ELAV like 1, ELAV like RNA binding protein 1, ELAV-like protein 1, ELAV1, ELAV1, Elavl1, Embryonic lethal abnormal vision drosophila homolog like 1, Hu Antigen R, Hu-antigen R, Hua, HuR, MelG,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

Applications:

WB IHC IP FC

Usage:

WB:1:500-1:2,000 IHC:1:100-1:500 FC:1:50-1:100 IP:1:10-1:50

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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