Background:

In mammalian cells, translation is controlled at the level of polypeptide chain initiation by eukaryotic initiation factors. The human eukaryotic translation initiation factor 5A gene, also designated eIF-4D or eIF5A, maps to chromosome 17p13.1 and encodes a 154 amino acid protein that is linked to cellular polyamine homeostasis. eIF5A localizes to the nuclear and cytoplasmic compartments of mammalian cells where it can stimulate ribosomal peptidyl-transferase and may be involved in nucleocytoplasmic mRNA transport and/or protein translation. eIF5A contains a unique spermidine-derived post-translational modification at Lys-50, hypusine, which is necessary for eIF5A's biochemical activity and for cellular proliferative signaling. In addition, eIF5A is a cellular cofactor for the function of the Rev transactivator protein of human immunodeficiency virus type 1 (HIV-1). Inhibition of eIF5A interaction with Rev leads to a block of the viral replication cycle.

Specificity:

eIF5A polyclonal antibody detects endogenous levels of eIF5A protein.

Alternative names:

eIF-5A-1, eIF-5A1, eIF-5A, EIF5A, eIF 4D, eIF 5A 1, EIF 5A, eIF 5A1, eIF-4D, eIF-5A, eIF-5A-1, eIF-5A1, eIF4D, eif5a, EIF5A1, eIF5AI, Eukaryotic initiation factor 5A, Eukaryotic initiation factor 5A isoform 1, Eukaryotic translation initiation factor 5A 1, Eukaryotic translation initiation factor 5A, Eukaryotic translation initiation factor 5A-1, IF5A1, MGC104255, MGC99547, Rev binding factor, Rev-binding factor, uORF A, uORF,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

18 kDa

Applications:

WB ICC IHC IP FC

Usage:

WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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