Background:

A key element in the pathway involved in the transduction of signals from activated protein-tyrosine kinase transmembrane receptors has been identified as the family of mitogen-activated protein kinases (MAP kinases). The most well known of these Ser/Thr kinases are ERK 1 and ERK 2. Mitogenic stimulation of cells triggers the activation of MAP kinases through phosphorylation of both tyrosyl (Y185) and threonyl (T183) residues. Phosphorylation of the T183 and Y185 ERK regulatory site is mediated by MAP kinase (MEK), which in turn is regulated by the proto-oncogene product Raf. Two highly related phosphatases, designated MKP-1 and MKP-2, exhibit 59% sequence identity at the amino acid level and oppose the action of MEK by downregulating the kinase activity of ERK 1 and ERK 2. MAP kinase phosphatase-1 and -2 proteins function by dephosphorylating ERK 1 and ERK 2 at their T-E-Y regulatory motif. An additional phosphatase encoded by the DUSP2 gene, designated PAC-1, also functions to downregulate ERK 1 and ERK 2 kinase activity. PAC-1 is a nuclear protein whose expression is strongly induced in response to mitogen.

Specificity:

DUSP1 polyclonal antibody detects endogenous levels of DUSP1 protein.

Alternative names:

MAP kinase phosphatase 1, MKP-1, CL 100, CL100, Dual Specificity Phosphatase 1, Dual specificity protein phosphatase 1, Dual specificity protein phosphatase hVH1, DUS1, DUSP 1, Dusp1, HVH1, MAP kinase phosphatase 1, Mitogen-activated protein kinase phosphatase 1, MKP-1, MKP1, Protein tyrosine phosphatase CL100, Protein-tyrosine phosphatase CL100, PTPN10, Serine/threonine specific protein phosphatase, VH1,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

40 kDa

Applications:

WB IHC IP FC

Usage:

WB:1:1,000 FC:1:50-1:100 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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