Background:

REDD-1, also designated DNA-damage-inducible transcript 4, dig2 or RTP801, is thought to function in the regulation of reactive oxygen species (ROS). REDD-1 expression has also been linked to apoptosis, Ab toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level. REDD-1 negatively regulates the mammalian target of Rapamycin (mTOR), a serine/threonine kinase often referred to as FRAP. It is crucial in the coupling of extra- and intracellular cues to FRAP regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness.

Specificity:

DDIT4 polyclonal antibody detects endogenous levels of DDIT4 protein.

Alternative names:

REDD-1, DDIT4, REDD1, RTP801, DDIT4, DDIT4, Dig2, DNA damage inducible transcript 4, DNA damage inducible transcript 4 protein, DNA damage-inducible transcript 4 protein, FLJ20500, HIF 1 responsive protein RTP801, HIF 1 responsive RTP801, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1, REDD-1, REDD1, RTP801,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Peptide.

Purification & Purity:

Peptide affinity purified

Molecular weight:

25 kDa (Predicted band size）

Applications:

WB ICC IHC FC

Usage:

WB:1:500 ICC:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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