Background:

Cleavage of the 5'-cap structure is involved in the major 5'-to-3' and nonsense-mediated mRNA decay pathways. The protein complex consisting of Dcp1 and Dcp2 has been identified as the species responsible for the decapping reaction in Saccharomyces cerevisiae. In nonsense-mediated decay, the human decapping complex, made up of S. cerevisiae homologs Dcp1a and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. hDcp2 specifically hydrolyzes methylated capped RNA to release m(7)GDP, thereby aiding in mRNA degradation. Both Dcp1a and hDcp2 colocalize in the cytoplasm. In addition, Dcp1a interacts with Smad4 forming a complex with TGFβ and BMP-4. Dcp1a and Smad4 interact directly through a EVH1/WH1 domain on Dcp1a and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of Dcp1a as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of Dcp1a eliminates TGFβ-induced nuclear translocation of Dcp1a.

Specificity:

Dcp1a polyclonal antibody detects endogenous levels of Dcp1a protein.

Alternative names:

DCP1A, SMIF, DCP1 decapping enzyme homolog A, Dcp1a, DCP1A, Decapping enzyme hDcp1a, Decapping mRNA 1A, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A, Nbla00360, Putative protein product of Nbla00360, Smad4 interacting transcriptional co activator, Smad4-interacting transcriptional co-activator, Smad4-interacting transcriptional co-activator, SMAD4IP1 antibody SMIF, Transcription factor SMIF,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Purification & Purity:

ProA affinity purified

Molecular weight:

63 kDa(Predicted band size)

Applications:

WB ICC IHC FC

Usage:

WB:1:500-1:2000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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