Background:

Activation of the cell surface receptor FAS by FAS ligand leads to the initiation of apoptosis, a process necessary for the regulation of the immune system and tissue homeostasis. FAS-mediated apoptosis appears to involve a number of divergent and overlapping pathways. Daxx appears to be a central component of a FAS-mediated apoptotic pathway involving the activation of Jun N-terminal kinase (JNK). Although Daxx itself does not contain a death domain, it specifically binds to the death domain of FAS. Overexpression of Daxx activates the JNK pathway and enhances FAS-mediated apoptosis. The Daxx apoptotic pathway acts cooperatively with but is distinct from the FAS-mediated pathway that involves interactions between the death domain-containing protein FADD and the cysteine protease FLICE. Unlike the FAS-FADD-FLICE pathway, the Daxx pathway is sensitive to the apoptotic inhibitor protein Bcl-2.

Specificity:

Daxx polyclonal antibody detects endogenous levels of Daxx protein.

Alternative names:

hDaxx, EAP1, BING 2, BING2, CENP-C binding protein, DAP 6, DAP6, DAXX, DAXX, Death associated protein 6, Death domain associated protein 6, Death domain associated protein, Death domain-associated protein 6, EAP 1, EAP1, ETS1 associated protein 1, ETS1-associated protein 1, Fas binding protein, Fas death domain associated protein, Fas death domain-associated protein, hDaxx, MGC126245, MGC126246,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

81 kDa

Applications:

WB ICC FC

Usage:

WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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