Background:

Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson's disease and drug abuse. DARPP-32 (for dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.

Specificity:

DARPP32 polyclonal antibody detects endogenous levels of DARPP32 protein.

Alternative names:

PPP1R1B, DARPP 32, DARPP-32, Dopamine and cAMP regulated neuronal phosphoprotein 32, Dopamine and cAMP regulated neuronal phosphoprotein, Dopamine and cAMP regulated phosphoprotein, Dopamine and cAMP regulated phosphoprotein DARPP 32, Dopamine and cAMP regulated phosphoprotein DARPP32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, IPPD, Neuronal phosphoprotein DARPP 32, PPP1R1B, PPR1B, Protein phosphatase 1 regulatory (inhibitor) subunit 1B, Protein phosphatase 1 regulatory inhibitor subunit 1B, Protein phosphatase 1 regulatory subunit 1B,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

32 kDa

Applications:

WB ICC/IF IHC IP FC

Usage:

WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

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