Background:

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. They have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 8 expression is seen in epithelium and epithelium-derived tumors. The Cytokeratin 8 and 18 pair are normally expressed in simple epithelia, but not in stratified epithelial cells. Research indicates that squamous cell carcinomas derived from stratified epithelia show abnormal expression of Cytokeratin 8 and 18, although it is not known whether these proteins contribute to the malignant phenotype of the cells. Expression of Cytokeratin 8 and 18 in oral squamous cell carcinomas is an independent prognostic marker that indicates a poor prognosis. Cytokeratin 8 expression correlates with malignancy in leukoplakia and carcinomas of the head and neck; it is expressed in all non-small-cell lung cancers. Cytokeratin 8 has been shown to possess extracellular epitopes on tumor cells, which may represent valuable targets for therapy.

Specificity:

Cytokeratin 8/18 polyclonal antibody detects endogenous levels of Cytokeratin 8/18 protein.

Alternative names:

CK-8, K8, KRT8, CYK8, CK-18, K18, KRT18, CYK18, CARD2, Cell proliferation inducing gene 46 protein, Cell proliferation inducing protein 46, CK 8, CK-8, CK18, CK8, CYK18, CYK8, Cytokeratin 18, Cytokeratin 8, Cytokeratin-8, K18, K2C8, K2C8, K8, Keratin 18, Keratin 8, Keratin, Keratin type I cytoskeletal 18, keratin type II cytoskeletal 8, Keratin-8, KO, KRT18, KRT8, type II cytoskeletal 8, Type-II keratin Kb8,

Species reactivity:

Human, Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

53 kDa

Applications:

WB ICC/IF IHC IP FC

Usage:

WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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