Background:

Cystatin C is a cysteine (thiol) protease inhibitor that belongs to the type II cystatin gene superfamily and is the most abundant extracellular inhibitor of cysteine proteases. Cystatin C is a constitutively secreted, amyloidogenic protein, which forms a two-fold symmetric dimer and modulates both cysteine protease activity and the expression of class II MHC molecules. Expression of cystatin C is an indicator of kidney function and glomerular filtration rate. Mutations in the cystatin C gene can lead to protein aggregates, which are implicated in hereditary amyloid angiopathy (HCCAA) and cerebral hemorrhage. Although both wild-type and mutant cystatin C are capable of forming concentration dependent inactive dimers, mutant cystatin C dimerizes at lower concentrations and is more susceptible to serine proteases, which may facilitate aggregation. In neuronal cells, oxidative stress stimulates expression of cystatin C, which may positively regulate apoptosis.

Specificity:

Cystatin C polyclonal antibody detects endogenous levels of Cystatin C protein.

Alternative names:

CST3, AD 8, AD8, Amyloid angiopathy and cerebral hemorrhage, ARMD11, bA218C14.4 (cystatin C), bA218C14.4, Cst 3, Cst3, CST3 protein, Cystatin 3, Cystatin-3, Cystatin-C, Cystatin3, CystatinC, CYTC, Epididymis secretory protein Li 2, Gamma trace, Gamma-trace, HCCAA, HEL S 2, MGC117328, Neuroendocrine basic polypeptide, Post gamma globulin, Post-gamma-globulin,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

16 kDa

Applications:

WB IHC

Usage:

WB:1:1,000-1:2,000 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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