Background:

P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endo-genous and xenobiotic compounds. P450 enzymes can be classified, based on their sequence similarities, into distinct subfamilies, which include CYP1A and CYP2A. Other P450 family members include CYP19, also designated aromatase (P450arom), which catalyzes the conversion of C19 steroids to estrogens in various tissues, including placenta, gonads, adipose tissue, skin and brain. CYP19 expression is controlled by hormonally regulated promoters in different tissues and increased aromatase activity is associated with familial gynecomastia. Also, a polymorphic allele of CYP19 (repeat (TTTA)12) is present in a majority of breast cancer patients. P450 cholesterol 7a-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver.

Specificity:

CYP27A1 polyclonal antibody detects endogenous levels of CYP27A1 protein.

Alternative names:

CYP27A1, CYP27, 12-alpha-triol 27-hydroxylase, 5-beta-cholestane-3-alpha, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase, 7-alpha, Cholestanetriol 26 monooxygenase, CP27, CP27A, CTX, CYP, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P-450C27/25, Cytochrome P450 27, Cytochrome P450 27 mitochondrial, Cytochrome P450 family 27 subfamily A member 1, Cytochrome P450 family 27 subfamily A polypeptide 1, Cytochrome P450 subfamily XXVIIA (steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1, mitochondrial, Sterol 26 hydroxylase, Sterol 26 hydroxylase mitochondrial, Sterol 26-hydroxylase, Sterol 27 hydroxylase, Sterol 27-hydroxylase, Vitamin D(3) 25 hydroxylase, Vitamin D(3) 25-hydroxylase,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein corresponding to human CYP27A1 300-500aa.

Purification & Purity:

ProA affinity purified

Molecular weight:

60 kDa

Applications:

WB ICC IHC FC

Usage:

WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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