Background:

Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD(+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity. This antibody recognizes the apoptosis-specific 89 kDa catalytic domain fragment, but it does not recognize the full-length PARP-1 or the 24 kDa DNA binding domain fragment.

Specificity:

Cleaved PARP polyclonal antibody detects endogenous levels of Cleaved PARP protein.

Alternative names:

PARP-1, ARTD1, ADPRT 1, PARP1, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT 1, ADPRT, ADPRT1, APOPAIN, ARTD1, NAD(+) ADP-ribosyltransferase 1, PARP, PARP-1, PARP1, PARP1, Poly [ADP-ribose] polymerase 1, Poly ADP ribose polymerase 1, Poly[ADP-ribose] synthase 1, PPOL, SCA1,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

89 kDa

Applications:

WB ICC IP FC

Usage:

WB:1:1,000-1:2,000 FC:1:50-1:100 ICC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com