Background:

Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA).

Specificity:

c-Jun polyclonal antibody detects endogenous levels of c-Jun protein.

Alternative names:

AP1, JUN, Activator protein 1, AP 1, AP1, cJun, Enhancer Binding Protein AP1, Jun Activation Domain Binding Protein, JUN, Jun oncogene, JUN protein, Jun proto oncogene, JUN, JUNC, Oncogene JUN, p39, Proto oncogene c jun, Proto oncogene cJun, Proto-oncogene c-jun, Transcription Factor AP 1, Transcription factor AP-1, Transcription Factor AP1, V jun avian sarcoma virus 17 oncogene homolog, V jun sarcoma virus 17 oncogene homolog (avian), V jun sarcoma virus 17 oncogene homolog, V-jun avian sarcoma virus 17 oncogene homolog, vJun Avian Sarcoma Virus 17 Oncogene Homolog,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

43 kDa

Applications:

WB ICC IHC IP

Usage:

WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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