Background:

Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires additional phosphorylation at Thr 160. The enzyme responsible for the phosphorylation of Cdk2 on Thr 160 and also of Cdc2 p34 on Thr 161, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit and a regulatory subunit. The catalytic subunit, designated Cdk7, has been identified as the mammalian homolog of MO15, a protein kinase demonstrated in starfish and Xenopus. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. Like other Cdks, Cdk7 contains a conserved threonine residue required for full activity; mutation of this residue severely reduces CAK activity.

Specificity:

Cdk7 polyclonal antibody detects endogenous levels of Cdk7 protein.

Alternative names:

p39 Mo15, CDK7, 39 kDa protein kinase, CAK, CAK1, Cdk activating kinase, CDK-activating kinase 1, CDK-activating kinase, cdk7, CDK7, CDKN7, Cell division protein kinase 7, Cyclin dependent kinase 7, cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase), Cyclin-dependent kinase 7, HCAK, Homolog of Xenopus MO15 Cdk activating kinase, Kinase subunit of CAK, MO15, MO15, Xenopus, homolog of, P39 Mo15, p39MO15, Serine threonine kinase Stk1, Serine/threonine protein kinase 1, Serine/threonine protein kinase MO15, STK1, TFIIH basal transcription factor complex kinase subunit,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

39 kDa

Applications:

WB ICC/IF IHC FC

Usage:

WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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