Background:

Cell cycle events are regulated by the sequential activation and deactivation of cyclin-dependent kinases (Cdks), including Cdk2 and Cdc2. Cdk2 in complexes with cyclin E and cyclin A appears necessary for the onset and progression of DNA replication, while the Cdc2 kinase in complexes with cyclin A or cyclin B is required for the initiation of cell division. Wee 1 has been identified as a protein kinase that suppresses the entry into mitosis by mediating inhibiting tyrosine phosphorylation of Cdc2 p34. In contrast, members of the Cdc25 family of protein phosphatases function as mitotic activators by dephosphorylation of Cdc2 p34 on regulatory tyrosine and possibly threonine residues. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.

Specificity:

Cdc25C polyclonal antibody detects endogenous levels of Cdc25C protein.

Alternative names:

CDC25C, CDC 25, Cdc 25C, CDC25, CDC25C, Cell division cycle 25 homolog C, Cell division cycle 25C, Cell division cycle 25C protein, Dual specificity phosphatase Cdc25C, M phase inducer phosphatase 3, M-phase inducer phosphatase 3, Mitosis inducer CDC25, MPIP3, MPIP3, Phosphotyrosine phosphatase, PPP1R60, protein phosphatase 1, regulatory subunit 60,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Peptide

Purification & Purity:

Peptide affinity purified.

Molecular weight:

53 kDa

Applications:

WB ICC IHC FC

Usage:

WB:1:500-1:1,000 ICC:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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