Background:

The transcription factor Polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (Osteoblast-specific transcription factor), CBFA1 (Core Binding Factor) and AML3 (Acute myeloid leukemia), is composed of two subunits, a and b, which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2a subunits, PEBP2aA, PEBP2aB and PEBP2aC, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, Runt. This region is involved in DNA binding and heterodimerization with the regulatory b subunit, which facilitates DNA binding of the a subunit. Both subunits are required for in vivo function; the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2b is the target of chromosomal inversion 16 (p13;q22) with the smooth muscle myosin heavy chain, producing a chimeric gene, PEBP2b/CBFB-SMMHC, that is associated with human acute myeloid leukemia.

Specificity:

CBFb polyclonal antibody detects endogenous levels of CBFb protein.

Alternative names:

CBF-beta, PEA2-beta, PEBP2-beta, CBFB, CBF b, CBF beta, CBF-beta, CBFB, CBFbeta, Core binding factor beta subunit, core binding factor subunit beta, Core-binding factor subunit beta, PEA 2, PEA2, PEA2 beta, PEA2-beta, PEA2beta, PEBB, PEBP 2B, PEBP2 beta, PEBP2-beta, PEBP2B, PEBP2beta, Polyomavirus enhancer binding protein 2 beta subunit, Polyomavirus enhancer-binding protein 2 beta subunit, SL3 3 enhancer factor 1 beta subunit, SL3 3 enhancer factor 1 subunit beta, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core binding factor beta subunit, SL3/AKV core-binding factor beta subunit,

Species reactivity:

Human, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within human CBFb aa 1-200.

Purification & Purity:

ProA affinity purified

Molecular weight:

22 kDa

Applications:

WB IHC FC

Usage:

WB:1:500 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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