Background:

The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177.

Specificity:

CAMKIV polyclonal antibody detects endogenous levels of CAMKIV protein.

Alternative names:

CaMK IV, CAMK4, CAMK, CAMK-GR, CAMKIV, Brain Ca(2+) calmodulin dependent protein kinase type 4, Brain Ca(2+) calmodulin dependent protein kinase type IV, Brain Ca++-calmodulin dependent protein kinase type IV, Calcium / calmodulin dependent protein kinase type 4 catalytic chain, Calcium / calmodulin dependent protein kinase type IV catalytic chain, Calcium/calmodulin dependent protein kinase IV, Calcium/calmodulin dependent protein kinase type IV, Calcium/calmodulin-dependent protein kinase type IV, CAM kinase 4, CAM kinase GR, CAM kinase IV, CAM kinase-GR, CaMK 4, CAMK GR, CaMK IV, Camk4, CaMKGR, IV, KCC4, MGC36771,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein corresponding to the C-terminus of human CAMKIV.

Purification & Purity:

ProA affinity purified

Molecular weight:

52 kDa

Applications:

WB ICC IF IHC FC

Usage:

WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com