Background:

In Drosophila, Polycomb (Pc-g) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. Mel-18 and Bmi-1, mammalian homologs of Drosophila Pc-g group proteins, are similarly expressed during development and implicated in the regulation of gene expression, axial skeleton development, control of proliferation and survival of haematopoietic cells. Mel-18 directly binds to DNA through a RING-finger motif and preferentially associates with juxtaposed enhancer elements on various genes, including Bcl-2, c-Myc and Hox. Mel-18 is an immediate early response gene within the c-Myc/Cdc25 signaling cascade that exhibits tumor suppressor activity and negatively regulates cell cycle progression by blocking S phase entry. Alternatively, Bmi-1 has been identified as a potent oncogene as it contributes to the transcriptional activation of genes implicated in early lymphoid development. Proviral activation of Bmi-1 expression corresponds to enhanced gene-specific activation of other proto-oncogenes, including c-Myc and Pim, subsequently resulting in the progression of lymphomagenesis.

Specificity:

Bmi1 polyclonal antibody detects endogenous levels of Bmi1 protein.

Alternative names:

BMI1, B lymphoma Mo MLV insertion region (mouse), B lymphoma Mo MLV insertion region 1 homolog, Bmi 1, BMI1, BMI1 polycomb ring finger oncogene, BMI1, Flvi 2/bmi 1, FLVI2/BMI1, MGC12685, Murine leukemia viral (bmi 1) oncogene homolog, Oncogene BMI 1, PCGF 4, PCGF4, Polycomb complex protein BMI 1, Polycomb complex protein BMI-1, Polycomb group protein Bmi1, Polycomb group ring finger 4, Polycomb group RING finger protein 4, RING finger protein 51, RNF 51, RNF51,

Species reactivity:

Human, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

42 kDa

Applications:

WB ICC/IF IHC

Usage:

WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com