Background:

Transcriptional activator which forms a core component of the circadian clock. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. ARNTL/BMAL1 positively regulates myogenesis and negatively regulates adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway.

Specificity:

BMAL1 polyclonal antibody detects endogenous levels of BMAL1 protein.

Alternative names:

bHLHe5, ARNTL, BHLHE5, BMAL1, MOP3, PASD3, ARNT like protein 1 brain and muscle, Arntl, Aryl hydrocarbon receptor nuclear translocator like, Aryl hydrocarbon receptor nuclear translocator like protein 1, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic helix loop helix PAS orphan MOP3, Basic helix loop helix PAS protein MOP3, Basic-helix-loop-helix-PAS protein MOP3, bHLH PAS protein JAP3, bHLH-PAS protein JAP3, bHLHe5, BMAL 1, BMAL1, BMAL1c, Brain and muscle ARNT like 1, Brain and muscle ARNT-like 1, CG8727 PA, Class E basic helix-loop-helix protein 5, cycle, JAP 3, JAP3, Member of PAS protein 3, Member of PAS superfamily 3, MGC47515, MOP 3, MOP3, PAS domain-containing protein 3, PASD 3, PASD3, TIC,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

68 kDa

Applications:

WB ICC/IF

Usage:

WB:1:1,000-1:2,000 ICC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com