Background:

The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18) bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. A second protein, designated Bcl-associated X protein (Bax) p21, has extensive amino acid homology with Bcl-2 and both homodimerizes and heterodimerizes with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3-dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Bcl-x, one of several additional proteins with sequence homology to Bcl-2, is expressed as Bcl-xL, a 233 amino acid protein with 43% sequence identity with Bcl-2 that suppresses cell death, and Bcl-xS, a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188) found in Bcl-xL and which functions as a dominant inhibitor of Bcl-2. A further apoptosis-inducing protein, Bad, dimerizes both with Bcl-xL and to a lesser extent with Bcl-2, thus displacing Bax and inducing apoptosis.

Specificity:

Bcl-XL polyclonal antibody detects endogenous levels of Bcl-XL protein.

Alternative names:

Bcl2-L-1, BCL2L1, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Apoptosis regulator BclX, B cell lymphoma 2 like, B2CL1, Bcl 2 like 1 protein, Bcl X, Bcl xL, BCL XL/S, Bcl xS, Bcl-2-like protein 1, Bcl2 Like 1, Bcl2 related gene, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, DKFZp781P2092, PPP1R52, Protein phosphatase 1 regulatory subunit 52,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

26 kDa

Applications:

WB ICC/IF IHC IP FC

Usage:

WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com