Background:

The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53. The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors. Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation. Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression. 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus. The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus. 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65.

Specificity:

ASPP2 polyclonal antibody detects endogenous levels of ASPP2 protein.

Alternative names:

TP53BP2, Bbp, 53BP2, p53BP2, 53BP2, Apoptosis stimulating of p53 protein 2, Apoptosis stimulating protein of p53 2, Apoptosis-stimulating of p53 protein 2, ASPP2, ASPP2, BBP, Bcl2 binding protein, Bcl2-binding protein, NY REN 51 antigen, p53 binding protein 2, p53-binding protein 2, p53BP2, PPP1R13A, Renal carcinoma antigen NY-REN-51, Tp53bp2, Tumor protein p53 binding protein 2, Tumor suppressor p53 binding protein 2, Tumor suppressor p53-binding protein 2,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

150 kDa

Applications:

WB ICC/IF IHC

Usage:

WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com