Background:

The AMBP (α-1-Microglobulin/bikunin precursor) gene encodes a protein precursor, known as AMBP, that is cleaved to produce two distinct proteins, designated a-1-Microglobulin and Bikunin. α-1-Microglobulin, also known as protein HC, is a member of the lipocalin superfamily and is secreted mainly in plasma, urine and cerebrospinal fluid. Thought to have reductase/dehydrogenase activity, α-1-Microglobulin exhibits immunosuppressive properties, such as cytokine secretion and inhibition of antigen-induced lymphocyte cell proliferation, and may be involved in the reduction of biological pro-oxidants. The second protein cleavage product, designated Bikunin and also known as inter-α-trypsin inhibitor light chain, ITI-LC or urinary trypsin inhibitor, is a widely expressed protein that is stored in the granules of human connective tissue mast cells. One of many proteins in the Kunitz-type protease inhibitor family, Bikunin prevents autodigestion by exocrine enzymes, such as trypsinogen and chymo-trypsinogen, and plays a role in the antiinflammatory/antiproteinase immune response. Unlike α-1-Microglobulin, Bikunin is implicated in the pathogenesis of a number of renal diseases, such as urolithiasis.

Specificity:

AMBP polyclonal antibody detects endogenous levels of AMBP protein.

Alternative names:

Protein HC, ITI-LC, AMBP, HCP, ITIL, Alpha 1 microglobulin/bikunin precursor, Alpha-1 microglycoprotein, AMBP, AMBP, Bikunin, Complex-forming glycoprotein heterogeneous in charge, EDC1, Growth inhibiting protein 19, HI 30, HI-30, HI30, IATIL, Inter alpha trypsin inhibitor light chain, ITI, ITI LC, ITI-LC, ITILC, Protein HC, Trypstatin, Uronic-acid-rich protein, UTI,

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein with Human AMBP aa 120-320.

Purification & Purity:

Protein affinity purified.

Molecular weight:

39 kDa (Predicted band size)

Applications:

WB ICC IHC FC

Usage:

WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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