Background:

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ and η that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Specificity:

14-3-3 epsilon polyclonal antibody detects endogenous levels of 14-3-3 epsilon protein.

Alternative names:

14-3-3E, YWHAE, 14 3 3 E, 14 3 3 epsilon, 14 3 3E, 14-3-3 protein epsilon, 14-3-3E, 1433E, Epididymis luminal protein 2, FLJ45465, FLJ53559, HEL2, KCIP 1, KCIP1, MDCR, MDS, Mitochondrial import stimulation factor L subunit, Protein kinase C inhibitor protein1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide, YWHAE,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

29 kDa

Applications:

WB ICC/IF IHC FC

Usage:

WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com