Background:

RNA polymerase II (RNAPII) holoenzyme contains numerous proteins that largely consist of RNA processing factors, RNA helicase, general transcription factors and SRB co-activating complexes. RNAPII mediated basal- and gene-specific transcriptional activation requires the association of various cofactors that includes PC4 (human Positive cofactor 4). Positive cofactor 4 interacts with the activation domain of transcription factor IIA (TFIIA) and TATA-binding protein (TBP)-associated factors (TAFs) to directly bind to double stranded DNA. Positive cofactor 4 induces both activation and repression of RNAPII basal transcription, depending on the presence or absence of these transcription factors and holoenzyme components. Addition-ally, Positive cofactor 4 is phosphorylated by TFIID and TFIIH, which releases Positive cofactor 4 from the DNA promoter region and thereby inhibits the assembly of Positive cofactor 4 into the transcriptional promoting complex and blocks transcription.

Specificity:

SUB1 polyclonal antibody detects endogenous levels of SUB1 protein.

Alternative names:

Activated RNA polymerase II transcriptional coactivator p15; Positive cofactor 4; PC4; SUB1 homolog; p14; SUB1; PC4, RPO2TC1

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human SUB1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 14kDa

Applications:

WB,IHC,IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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