Background:

EF-Ts, also known as TSFM (Ts translation elongation factor, mitochondrial) or COXPD3, is a 325 amino acid protein that is one of 13 mitochondrial-encoded proteins that work together during the elongation phase of protein biosynthesis on the ribosome. Expressed ubiquitously with highest levels present in liver, kidney and skeletal muscle, EF-Ts associates with EF-Tu, a multidomain GTPase with essential functions in translation, and, via this interaction, facilitates the exchange of GDP for GTP, thereby inducing protein elongation. Mutations in the gene encoding EF-Ts are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3), a condition characterized by defects in the mitochondrial oxidative phosphorylation system and often characterized by severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Multiple isoforms of EF-Ts exist due to alternative splicing events.

Specificity:

EF-Tu polyclonal antibody detects endogenous levels of EF-Tu protein.

Alternative names:

Elongation factor Tu, mitochondrial; EFTu; P43; TUFM

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human EF-Tu.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 49kDa

Applications:

WB,IHC,IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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