Background:

Transgelin (also designated SM22α), is expressed abundantly in smooth muscle cells. Transgelin-2 (also known as SM22α homolog) is a homolog of transgelin and is also expressed in smooth muscle cells and by peritoneal B-1 cells. The human transgelin-2 gene (designated TAGLN2), which is located on chromosome 1q231-q25, encodes a 199 amino acid protein that contains a calponin-like repeat and a calponin-homology (CH) domain. Transgelin-2 may function very similarly to transgelin. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is downregulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds actin, causing actin fibers to gel within minutes of binding. Binding of transgelin to actin occurs at a ratio of 1:6 actin monomers.

Specificity:

TAGLN2 polyclonal antibody detects endogenous levels of TAGLN2 protein.

Alternative names:

Transgelin-2; Epididymis tissue protein Li 7e; SM22-alpha homolog; TAGLN2; KIAA0120; CDABP0035

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human TAGLN2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 22 kDa

Applications:

WB IF

Usage:

WB: 1:500~1:2000 IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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